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3D Cell Culture Kits Greiner Bio-One

Magnetic 3D Cell Culture

The core technology of Greiner Bio-One’s Magnetic 3D Cell Culture is the magnetisation of cells with NanoShuttle™-PL. The cells can be aggregated with magnetic forces, either by levitation or printing, to form structurally and biologically representative 3D models in vitro. NanoShuttle™-PL consists of gold, iron oxide, and Poly-L-Lysine. These nanoparticles (Ø < 50 nm) magnetise cells by electrostatically attaching
to cell membranes during an overnight static incubation. Magnetised cells will appear peppered with dark nanoparticles after incubation. NanoShuttle™-PL is biocompatible, having no effect on metabolism, proliferation and inflammatory stress. Additionally, it does not interfere with experimental techniques,
such as fluorescence or Western blotting. With magnetised spheroids, solution addition and removal is made easy by using magnetic force to hold them in a stationary position during aspiration, thereby limiting spheroid loss. Spheroids can also be picked up and transferred between vessels using magnetic tools such as the MagPen™ (7.657 850).


Advantages of magnetic cell culture:
  • 3D in a 2D workflow
  • Fast 3D tissue assembly
  • Easy to handle / no sample loss
  • Scalable – single well to 384 well
  • Ease of co-culturing cells
  • High-throughput Screening (HTS) with flat bottom for optical imaging
  • Ready for automation
  • Positioning spheroid centrally within the well


Magnetic Levitation
Magnetic levitation is an easy tool to create native tissue environments in vitro. Cells are magnetised with
NanoShuttle™-PL through overnight incubation and dispensed into a cell-repellent dish or multiwell plate, where they are levitated off the bottom by a magnet above the cell-repellent vessel. When levitating, cells come off the stiff well bottom and the magnetic forces work as an invisible scaffold that rapidly aggregates cells to encourage cell-cell interactions and induce ECM synthesis. The magnetic levitation method has been successfully used to make 3D cultures with different cell types, including cell lines, stem cells and primary cells. The basic application of this technology is to culture 3D cell cultures under different environmental conditions and then analyse them using common biological research techniques, such as
immunohistochemical analysis and western blotting.


Magnetic Bioprinting
In contrast to magnetic levitation, with magnetic 3D bioprinting, cells incubated with NanoShuttle™-PL overnight are printed into spheroids by placing a microplate containing magnetised cells atop a drive of magnets. The magnets below each well utilise mild magnetic forces to induce cell aggregation and print a spheroid at the bottom of each well. After 15 minutes to a few hours, the plate containing the spheroids can be removed from the magnetic drive and cultured long-term without use of magnetic force. This
system overcomes the limitations of other platforms by enabling rapid formation of spheroids unattached to any stiff substrate, reproducible in size without limitation to cell type and scalable in size for high-throughput formats (96 and 384 well). With magnetic 3D bioprinting, viable spheroids can be printed that compact and subsequently grow over time, facilitating continuous assessment of cell viability and other functions using commercially available assays. The 3D printing method, together with commercially
available standardised assay methods, provide an ideal combination for high-throughput compound screening.


Magnetic Printing of Rings and Imaging System
Additionally, it was demonstrated that magnetic 3D bioprinting can be used to develop novel migration assays (ring formation) and that automated kinetic imaging can facilitate high-throughput screening. These assays form a foundation for robust screening of compound effects on cell migration. Based on magnetic 3D bioprinting, cells magnetised with NanoShuttle™-PL are printed into 3D rings. Immediately after printing, these structures will compact and eventually close, as a function of cell migration and viability. Ring closure can be captured using a compact imaging kit with an iPod*) programmed by a freely available app (Experiment Assistant) to image whole plates at specific intervals, foregoing the need
to image well-by-well under a microscope (Fig. 4). Culture contraction is generally complete within 24 hours, and images are batch processed to rapidly yield toxicity data. Moreover, as closure is label-free, the remaining rings are available for further experimentation (IHC, Western blot, genomics, etc.).
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item number description price
7.657 810 Consumables / Accessories for 3D Cell Culture Kits Greiner Bio-One from CHF 444.52
item number type no. description   price stock amount
7.627 830
Single-Well Drives • Single-Well Levitating Drives (3)
• Single-Well Concentrating Drives (3)

CHF 1'329.31

 

7.627 840
Link GBO
Single-Well Bio-Assembler Kit • 600 μl vials of NanoShuttle-PL (2)
• Single-Well Levitating Drives (3)
• Single-Well Concentrating Drives (3)
• 35 mm Cell Culture Dishes with Cell-Repellent Surface (10)
• Holding lid (1)

CHF 1'523.45

 

7.655 830
96-Well Drives • 96-Well Spheroid Drive (1)
• 96-Well Holding Drive (1)

CHF 1'690.01

 

7.655 840
96-Well Bioprinting Kit • 600 μl Vials of NanoShuttleTM-PL (3)
• 96-Well Spheroid Drive (1)
• 96-Well Holding Drive (1)
• 96 Well Cell Culture Microplate (Clear) with Cell-Repellent Surface (2)

CHF 2'216.22

 

7.655 841
96-Well Bioprinting Kit - Black μClear® Plates • 600 μl Vials of NanoShuttle-PL (3)
• 96-Well Spheroid Drive (1)
• 96-Well Holding Drive (1)
• 96 Well Cell Culture Microplate (Black, with μClear® Film Bottom) with Cell-Repellent Surface (2)

CHF 2'216.22

 

7.655 846
96-Well BiO Assay Kit • 600 μl Vials of NanoShuttle-PL (3)
• 6-Well Levitating Drive (1)
• 96-Well Spheroid Drive (1)
• 96-Well Holding Drive (1)
• 96-Well Ring Drive (1)
• 96-Well Deep Well Plate (1)
• 6-Well Cell Culture Multiwell Plates with Cell-Repellent Surface (2)
• 96-Well Cell Culture Microplates with Cell-Repellent Surface (2)

CHF 4'155.55

 

7.655 848
96-Well BiO Assay (Greiner Bio-One version) • 600 μl Vials of NanoShuttle-PL (3)
• 6-Well Levitating Drive (1)
• 96-Well Spheroid Drive (1)
• 96-Well Holding Drive (1)
• 96-Well Ring Drive (1)

CHF 4'017.63

 

7.655 849
96-Well BiO Assay Kit & n3Dock Imaging System • 600 μl Vials of NanoShuttle-PL (3)
• 6-Well Levitating Drive (1)
• 96-Well Spheroid Drive (1)
• 96-Well Holding Drive (1)
• 96-Well Ring Drive (1)
• 96-Well Deep Well Plate (1)
• 6-Well Cell Culture Multiwell Plates with Cell-Repellent Surface (2)
• 96-Well Cell Culture Microplates with Cell-Repellent Surface (2)
• n3Dock (1)
• Imaging Mask (1)
• Light Pad (1)
• Cooling Fan (1)
• AC-to-USB Adapter (1)
• Extension Cord (1)
• Analytical Software - link

CHF 6'786.58

 

7.657 830
6-Well Drives • 6-Well Levitating Drive (1)
• 6-Well Concentrating Drive (1)

CHF 1'190.33

 

7.657 840
6-Well Bio-Assembler Kit • 600 μl Vials of NanoShuttle-PL (2)
• 6-Well Levitating Drive (1)
• 6-Well Concentrating Drive (1)
• 6 Well Cell Culture Multiwell Plates with Cell-Repellent Surface (2)

CHF 1'523.45

 

7.662 824
24-Well Custom Lid • 3D printed special lid

CHF 665.18

 

7.662 830
24-Well Drives • 24-Well Levitating Drive (1)
• 24-Well Concentrating Drive (1)

CHF 1'384.47

 

7.662 840
24-Well Bio-Assembler Kit • 600 μl Vials of NanoShuttle-PL (2)
• 24-Well Levitating Drive (1)
• 24-Well Concentrating Drive (1)
• 24 Well Cell Culture Multiwell Plates with Cell-Repellent
• Surface (2)
• Custom Lid (1)

CHF 2'216.22

 

7.781 830
384-Well Drives • 384-Well Spheroid Drive (1)
• 384-Well Holding Drive (1)

CHF 3'739.67

 

7.781 840
384-Well Bioprinting Kit • 600 μl Vials of NanoShuttleTM-PL (2)
• 384-Well Spheroid Drive (1)
• 384-Well Holding Drive (1)
• 384-Well Cell Culture Microplate (Clear) with Cell-Repellent Surface (2)

CHF 4'016.57

 

7.781 841
384-Well Bioprinting Kit - Black μClear® Plates • 600 μl Vials of NanoShuttleTM-PL (2)
• 384-Well Spheroid Drive (1)
• 384-Well Holding Drive (1)
• 384-Well Cell Culture Microplate (Black, with μClear® Film Bottom) with Cell-Repellent Surface (2)

CHF 4'016.57

 

7.781 846
384-Well BiO Assay Kit • 600 μl Vials of NanoShuttle-PL (2)
• 6-Well Levitating Drive (1)
• 384-Well Spheroid Drive (1)
• 384-Well Holding Drive (1)
• 96-Well Deep Well Plate (1)
• 6-Well Cell Culture Multiwell Plates with Cell-Repellent Surface (2)
• 384-Well Cell Culture Microplates with Cell-Repellent Surface (2)

CHF 5'761.75

 

7.781 848
384-Well BiO Assay (Greiner Bio-One version) • 600 μl Vials of NanoShuttle-PL (2)
• 6-Well Levitating Drive (1)
• 384-Well Spheroid Drive (1)
• 384-Well Holding Drive (1)

CHF 5'623.83

 

7.781 849
384-Well BiO Assay Kit & n2Dock Imaging System • 600 μl Vials of NanoShuttle-Pl (2)
• 6-Well Levitating Drive (1)
• 384-Well Spheroid Drive (1)
• 384-Well Holding Drive (1)
• 96-Well Deep Well Plate (1)
• 6-Well Cell Culture Multiwell Plates with Cell-Repellent Surface (2)
• 384-Well Cell Culture Microplates with Cell-Repellent Surface (2)
• n3Dock (1)
• Imaging Mask (1)
• Light Pad (1)
• Cooling Fan (1)
• AC-to-USB Adapter (1)
• Extension Cord (1)
• Analytica Software - link

CHF 8'033.13

 

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