Cell Culture Dish Poly-L-Lysin Cellcoat® Greiner Bio-One

Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic molecules that are used to improve adhesion of different cell types to polystyrene surfaces (Fig. 1). Especially when serumfree or serum-reduced medium is used or when experiments such as transfections are performed, the cultivation efficiency of individual cell lines can be improved. As synthetic molecule Poly-D-Lysine is free of impurities carried by other proteins.

• Reduced-serum or serum-free cultivation
• Cell differentiation and neuron growth
• Promotion of cell adhesion, proliferation and growth of transfected cell lines (e.g. HEK 293, PC 12, L929, certain 3T3 cell lines), neuronal cell lines, as well as primary neurons and glia cells
• Synthetic polypeptides
• Molecular weight PDL: 75 – 150 kDa; PLL: 30 – 70 kDa
• Experiments with automated cell culture
• Experiments with washing steps
• Shelf life: 24 months (multiwell plates, dish) / 18 months (microplates) at room temperature
• Protein coating: Poly-L-Lysine
• With Lid