CELLine™ Bioreactors Wheaton

In an effort to push science forward, WHEATON manufactured a new line of membrane driven flasks for high density cell culture. The flasks are designed to enhance small scale bio-production for antibody and protein generation. Conventional in vivo or in vitro cell culture methods can be laborious, may have low cell density and require significant purification. CELLine flasks address these three areas of limitation observed in static tissue culture flasks.

How does the CELLine Flask Work?
Media Compartment – The media compartment allows for bulk storage of cell culture growth medium. This reduces the media refreshing requirement significantly as the media compartment is fifty times the size of the cell compartment
Metabolite Regulating Upper Membrane - The upper dialysis membrane has a 10 kDa cut off limit. This regulates the flow of metabolites to and from the cell compartment and retains all proteins in the cell compartment
Cell Compartment – The cell compartment provides the ideal area to inoculate and achieve high density cultures. The compartment concentrates cells, their products, and limits the requirement for any exogenous growth factors.
Gas Permeable Lower Membrane – With static cultures, gas transfer rates can be the limiting factor in high density cultures. The CELLine flask places the cells directly against the gas permeable membrane to achieve optimal levels of oxygen and carbon dioxide.

Benefits of CELLine Flask

• Disposable and ready-to-us
• High cell density and high product concentration
• Reduces operation time
• Decreases use of consumables
• Cost-efficient, space saving, and stackable
• No additional equipment required for operation